Nerve agent exposure elicits site-specific changes in protein phosphorylation in mouse brain

Changes in mouse liver protein glutathionylation after acetaminophen exposure.

The role of protein glutathionylation in acetaminophen (APAP)-induced liver injury was investigated in this study. A single oral gavage dose of 150 or 300 mg/kg APAP in B6C3F1 mice produced increased serum alanine aminotransferase and aspartate aminotransferase levels and liver necrosis in a dose-dependent manner. The ratio of GSH to GSSG was decreased in a dose-dependent manner, suggesting tha...

Effects of psychotropic drugs on nerve growth factor protein levels in the rat brain

Introduction: Psychotropic drugs exert their effects, in part, by increasing neurotrophin levels in the brain. Nerve growth factor (NGF) protein levels after treatment with only a limited number of psychotropics have been determined. The present study was designed in order to evaluate the effects of acute and chronic administration of different psychotropic drugs on NGF protein levels in fiv...

Accurate quantitation of protein expression and site-specific phosphorylation.

A mass spectrometry-based method is described for simultaneous identification and quantitation of individual proteins and for determining changes in the levels of modifications at specific sites on individual proteins. Accurate quantitation is achieved through the use of whole-cell stable isotope labeling. This approach was applied to the detection of abundance differences of proteins present i...

P48: Protein Changes Resulted in Sub-Chronic Neurotoxicity of Bisphenol A in Rat Brain

Bisphenol A (BPA) is one of the most widely used chemicals in the plastic industry, which enter the human body through occupational and food contact. In this study, the protein changes in rat cerebral cortex was evaluate in order to evaluate the neurotoxicity of BPA. 24 adult male rats were randomly selected and divided into four groups (n=6) and each group respectively received 0, 0.5, 5 and 5...

Pretreatment for Nerve Agent Exposure